Abstract Introduction: patients with granulomatosis with polyangiitis (GPA) may present upper airway (UA) and lower airway (LA) involvement. Objectives: we describe the endoscopic manifestations of the airways, histological findings from biopsied tissue and antineutrophilic cytoplasm antibody (ANCA) in a group of patients with GPA. Methods: retrospective study of medical records of patients with GPA undergoing bronchoscopy between 2012 and 2019. Airway findings, results of biopsies performed and ANCA results were analyzed. Results: 40 patients were included, with a mean age of 46.92±17.61 years and predominantly female (67.5%). Airway involvement was observed in 90% (n=36). The C-ANCA was reactive in 63.9%, P-ANCA in 25%, doubly reactive ANCA in 8.33% and non-reactive in 20%. The findings in upper airway (UA) were: chronic sinusitis (41.7%), destruction of the nasal septum (16.7%); and in lower airway (LA) were: tracheobronchial stenosis (38.9%) and tracheobronchitis (25%). The pathological findings most common of bronchoscopic biopsies were: polymorphonuclear inflammatory process (61.9%) and geographic necrosis (47.6%). Conclusion: the airway is involved in up to 90% of patients with GPA. Non-reactive ANCA does not rule out this possibility. Chronic sinusitis and tracheobronchial fibrostenotic processes were the most common endoscopic findings. Vasculitis in biopsies was found in a minority of cases.

Resumen Introducción: los pacientes con granulomatosis con poliangitis (GPA) pueden presentar compromiso de la vía aérea superior (VAS) o inferior (VAI). Objetivos: describimos las manifestaciones endoscópicas de las vías respiratorias, los hallazgos histológicos y los anticuerpos anticitoplasma de neutrófilos (ANCA) en un grupo de pacientes con GPA. Métodos: estudio retrospectivo de historias clínicas de pacientes con GPA sometidos a broncoscopia entre 2012 y 2019. Se analizaron hallazgos de la vía aérea, biopsias y ANCA. Resultados: se incluyeron 40 pacientes, con una edad media de 46,92±17,61 años, predominantemente del sexo femenino (67,5%). Se observó afectación de la vía aérea en el 90% (n=36). El C-ANCA fue reactivo en el 63,9%, P-ANCA en el 25%, ANCA doblemente reactivo en el 8,33% y no reactivo en el 20%. Los hallazgos comunes en la vía aérea superior (VS) fueron sinusitis crónica (41,7%), destrucción del tabique nasal (16,7%); y en la vía aérea inferior (AI): estenosis traqueobronquial (38,9%), traqueobronquitis (25%). Los hallazgos más frecuentes de las biopsias broncoscópicas fueron proceso inflamatorio polimorfonuclear (61,9%) y necrosis geográfica (47,6%). Conclusión: la vía aérea está comprometida hasta en un 90% de los pacientes con GPA. ANCA no reactivos no descartan esta posibilidad. La sinusitis crónica y los procesos fibroestenóticos traqueobronquiales fueron los hallazgos endoscópicos más comunes. La vasculitis en biopsias se encontró en una minoría de casos.

Abstract Objective To analyze the factors associated with children's linear growth, according to the different subsystems of the 6Cs model and Bronfenbrenner's Bioecological Theory. Data sources Narrative review, carried out in the Scielo, Lilacs, Pubmed, and Science Direct databases, based on research using the terms Bioecological Theory, child growth, and risk factors, combined with the use of Boolean operators. Summary of findings According to the 6Cs model, proposed based on Bronfenbrenner's Bioecological Theory, the determining factors of children's linear growth are in six interrelated subsystems - cell, child, clan, community, country/state and culture. The empirical studies included in this review made it possible to analyze protection and risk factors within the subsystems. Among the protective factors: are adequate birth weight and satisfactory weight gain, breastfeeding for six months or more; proper hygienic habit of hand washing, proper elimination of feces, and access to clean water. As risk factors: low, birth weight and size, prematurity, multiple deliveries, short interval between deliveries, non-exclusive breastfeeding until the 3rd month, frequency and severity of infectious processes and anemia, little parental education, short maternal statur, inadequate maternal nutritional status, domestic violence, family poverty, food, and nutritional insecurity, living in rural areas or at high altitudes. Conclusion Children's linear growth is determined by interrelated factors that encompass aspects prior to the child's birth, as well as socioeconomic, political, family and community issues.

Resumo O tétano é uma doença grave e rara em pequenos animais, de caráter agudo e mediada pela ação da neurotoxina tetanospasmina, proveniente do bacilo Clostridium tetani. Neste relato descreve-se um caso de tétano em um canino, com ênfase em seus aspectos clínicos e terapêuticos, além da sua recuperação clínica. Foi atendido no Hospital Veterinário da Universidade de Passo Fundo, um canino, fêmea, da raça Pitbull, com cinco meses, em status epilepticus. Após a estabilização da paciente, constataram-se animal em posição de cavalete, com paralisia espástica generalizada, risus sardonicus, trismo, cauda e orelhas eretas e dificuldade em expandir o tórax. O diagnóstico presuntivo de tétano foi firmado a partir da anamnese, sinais clínicos e achados laboratoriais. O tratamento suporte estabelecido incluiu reposição hidroeletrolítica, soro antitetânico, antibioticoterapia, analgesia, fármacos miorrelaxantes e anticonvulsivantes. O manejo intensivo da paciente foi realizado com sondagem uretral e nasoesofágica, trocas de decúbito a cada duas horas e diminuição de estímulos ambientais. A fisioterapia e a acupuntura também foram utilizadas para complementar o tratamento e acelerar a recuperação. A paciente recebeu alta médica um mês após o início do tratamento, apresentando evolução satisfatória. No presente caso, o exame clínico em associação aos sinais clínicos característicos da doença, acrescidos da anamnese minuciosa foram fundamentais ao diagnóstico presuntivo de tétano. Além disso, o manejo intensivo, o tratamento medicamentoso, assim como a realização de fisioterapia e acupuntura, possibilitaram a evolução do quadro clínico à cura.

Abstract Tetanus is a serious and rare disease in small animals, of an acute nature and mediated by the action of the neurotoxin tetanospasmin, from the bacillus Clostridium tetani. This report describes a case of tetanus in a canine, with emphasis on its clinical and therapeutic aspects, in addition to its clinical recovery. A canine, female, Pitbull breed, five months old, in status epilepticus, was attended at the Veterinary Hospital of the University of Passo Fundo. After stabilizing the patient, the animal was found in a trestle position, with generalized spastic paralysis, risus sardonicus, trismus, erect tail and ears, and difficulty in expanding the thorax. The presumptive diagnosis of tetanus was established based on the anamnesis, clinical signs, and laboratory findings. The established supportive treatment included hydro electrolytic replacement, antitetanus serum, antibiotic therapy, analgesia, myorelaxant and anticonvulsant drugs. The patient's intensive management was carried out with urethral and gastroesophageal probing, changes of position every two hours, and reduction of environmental stimuli. Physiotherapy and acupuncture were also used to complement the treatment and accelerate recovery. The patient was discharged one month after the beginning of the treatment, presenting satisfactory evolution. In the present case, the clinical examination in association with the characteristic clinical signs of the disease, added to the detailed anamnesis, was essential for the presumptive diagnosis of tetanus. In addition, intensive management, drug treatment, physiotherapy, and acupuncture enabled the evolution of the clinical condition to cure.

ABSTRACT Objective: to understand what undergraduate Nursing students from a university in southern Brazil know about trans people. Method: a descriptive study with a qualitative approach based on interviews conducted with 19 undergraduate Nursing students enrolled from the eighth to the tenth period of the course. Data collection took place in October and November 2021. Three thematic categories emerged from the thematic analysis, namely: the undergraduate course has to speak about this!; I don't know what it is about; and What is known and said about trans people. Results: the students showed insecurity in the care of trans people because this content is not regularly taught in the curriculum. They denote not knowing how to welcome, care for, respect specificities and treat them by their social name, as well as difficulties taking care of this population group, both in the present time and in the future. In addition to that, few of them had the opportunity of undergoing this experience in the academic practice fields. The content is taught in a single academic discipline that deals with sexuality in general. Conclusion: lack of knowledge about sexual diversity is a limiting factor in the health care provided to this population group. Certain weakness is identified in the teaching-learning process when not relating the reality of the trans population to their health demands.

RESUMEN Objetivo: comprender el conocimiento de los estudiantes de grado de Enfermería de una universidad del sur de Brasil en relación a las personas trans. Método: estudio descriptivo de enfoque cualitativo a partir de entrevistas realizadas con 19 estudiantes de grado de Enfermería inscritos en el octavo a décimo período del curso. La recolección de datos tuvo lugar en octubre y noviembre de 2021. Surgieron 3 categorías del análisis temático: ¡En la carrera de grado se debe hablar de esto!; No sé de qué se trata; y Lo que se sabe y dice sobre las personas trans. Resultados: los estudiantes se muestran inseguros en la atención de las personas trans debido a que este contenido no se ofrece regularmente en el plan de estudio. Denotan desconocimiento sobre como recibir a las personas trans, atenderlas, respetar sus especificidades y tratarlas por su nombre social, además de dificultades en el cuidado de este grupo poblacional, tanto en el presente como en el futuro. Además, pocos participantes tuvieron la oportunidad de vivir esta experiencia en los campos de práctica académica. El contenido solo se dicta en una disciplina que trata el tema dela sexualidad en general. Conclusión: el desconocimiento acerca de la diversidad sexual es un factor limitante en la atención de la salud que se proporciona a este grupo poblacional. Se identifica cierta fragilidad en el proceso de enseñanza-aprendizaje ya que no se relaciona la realidad de la población trans con sus necesidades en materia de salud.

RESUMO Objetivo: compreender o conhecimento dos estudantes de graduação de enfermagem em uma universidade do sul do Brasil no tocante às pessoas trans. Método: estudo descritivo com abordagem qualitativa a partir de entrevistas realizadas com 19 estudantes de graduação em enfermagem matriculados do oitavo ao décimo período do curso. A coleta de dados ocorreu nos meses de outubro e novembro de 2021. Da análise temática emergiram 3 categorias: o curso de graduação precisa falar disso!; eu não sei do que se trata; e o que se sabe e se diz sobre as pessoas trans. Resultados: os estudantes demonstram insegurança no cuidado das pessoas trans devido ao não oferecimento regular deste conteúdo no currículo. Denotam desconhecimento de como acolher, cuidar, respeitar as especificidades, tratar pelo nome social, e também dificuldades em cuidar desta população no presente e no futuro. Além disso, poucos tiveram a oportunidade de vivenciar esta experiência nos campos de prática acadêmica. O conteúdo é oferecido em uma única disciplina que trata da sexualidade em geral. Conclusão: o desconhecimento acerca da diversidade sexual é um fator limitador na assistência à saúde a esta população. Identifica-se uma fragilidade no ensino-aprendizagem ao não relacionar a realidade da população trans com suas demandas de saúde.

Resumo O tétano é uma doença grave e rara em pequenos animais, de caráter agudo e mediada pela ação da neurotoxina tetanospasmina, proveniente do bacilo Clostridium tetani. Neste relato descreve-se um caso de tétano em um canino, com ênfase em seus aspectos clínicos e terapêuticos, além da sua recuperação clínica. Foi atendido no Hospital Veterinário da Universidade de Passo Fundo, um canino, fêmea, da raça Pitbull, com cinco meses, em status epilepticus. Após a estabilização da paciente, constataram-se animal em posição de cavalete, com paralisia espástica generalizada, risus sardonicus, trismo, cauda e orelhas eretas e dificuldade em expandir o tórax. O diagnóstico presuntivo de tétano foi firmado a partir da anamnese, sinais clínicos e achados laboratoriais. O tratamento suporte estabelecido incluiu reposição hidroeletrolítica, soro antitetânico, antibioticoterapia, analgesia, fármacos miorrelaxantes e anticonvulsivantes. O manejo intensivo da paciente foi realizado com sondagem uretral e nasoesofágica, trocas de decúbito a cada duas horas e diminuição de estímulos ambientais. A fisioterapia e a acupuntura também foram utilizadas para complementar o tratamento e acelerar a recuperação. A paciente recebeu alta médica um mês após o início do tratamento, apresentando evolução satisfatória. No presente caso, o exame clínico em associação aos sinais clínicos característicos da doença, acrescidos da anamnese minuciosa foram fundamentais ao diagnóstico presuntivo de tétano. Além disso, o manejo intensivo, o tratamento medicamentoso, assim como a realização de fisioterapia e acupuntura, possibilitaram a evolução do quadro clínico à cura. animais tetanospasmina tetani descrevese descreve se canino terapêuticos clínica Fundo fêmea Pitbull meses epilepticus constataramse constataram cavalete generalizada sardonicus trismo tórax laboratoriais hidroeletrolítica antitetânico antibioticoterapia analgesia anticonvulsivantes nasoesofágica ambientais satisfatória disso medicamentoso cura

Abstract Tetanus is a serious and rare disease in small animals, of an acute nature and mediated by the action of the neurotoxin tetanospasmin, from the bacillus Clostridium tetani. This report describes a case of tetanus in a canine, with emphasis on its clinical and therapeutic aspects, in addition to its clinical recovery. A canine, female, Pitbull breed, five months old, in status epilepticus, was attended at the Veterinary Hospital of the University of Passo Fundo. After stabilizing the patient, the animal was found in a trestle position, with generalized spastic paralysis, risus sardonicus, trismus, erect tail and ears, and difficulty in expanding the thorax. The presumptive diagnosis of tetanus was established based on the anamnesis, clinical signs, and laboratory findings. The established supportive treatment included hydro electrolytic replacement, antitetanus serum, antibiotic therapy, analgesia, myorelaxant and anticonvulsant drugs. The patient's intensive management was carried out with urethral and gastroesophageal probing, changes of position every two hours, and reduction of environmental stimuli. Physiotherapy and acupuncture were also used to complement the treatment and accelerate recovery. The patient was discharged one month after the beginning of the treatment, presenting satisfactory evolution. In the present case, the clinical examination in association with the characteristic clinical signs of the disease, added to the detailed anamnesis, was essential for the presumptive diagnosis of tetanus. In addition, intensive management, drug treatment, physiotherapy, and acupuncture enabled the evolution of the clinical condition to cure. animals tetanospasmin tetani canine aspects recovery female breed old epilepticus Fundo paralysis sardonicus trismus ears thorax anamnesis findings replacement serum therapy analgesia drugs patients s probing hours stimuli physiotherapy cure

Background: The low available of Glut-4 transporters in sarcolemma of the cardiac cells is what characterizes the myocardial insulin resistance (MIR), which is triggered separately of generalized insulin resistance. Insulin receptors are quite evident in the heart muscle and vessels, and mitochondrial activity performs a significant function in MIR preserving cellular homeostasis by cell reproduction, cells livelihoods, and energy generation. Objective: To evaluate the MIR mechanism and through the signaling pathway design. Methods: PubMed database was employed to search for reviews publications with MIR. The referenced data of the signaling pathway was chosen aggregating references of the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. A signaling pathway was designed based on MIR research manuscripts, where we show several mechanisms included in the MIR. The KEGG server was employed to exploit the interrelationship protein-protein, and elaborate signaling pathway diagram. The signaling pathway mapping was carried out with PathVisio software. Results: We selected 42 articles from a total of 450 articles in the PubMed database that presented a significant association between the terms "insulin resistance myocardial" AND "signaling pathway". Founded on database-validated research papers, we choose well-founded pathways and we succeeded representative description of these pathways. The reproduction contigs taken from the KEGG database designed the signaling pathway of the bio-molecules that lead to MIR. Thus, the acting among multiple mechanisms releases factors that participate of the development of MIR. Conclusion: The interaction among various mechanisms and molecular interactions are important factors in development of MIR.

ABSTRACT Understanding the role of photosynthesis in the holoparasitic genus Cuscuta may give insights into the biology and evolution of these plants. We hypothesised that light irradiance on the node of C. racemosa is important for sustaining the growth of the new shoot through photosynthesis. We blocked light irradiance on the node of detached twigs of C. racemosa for one week and measured twig fresh and dry weight, shoot length and photosynthetic pigment content. Even though fresh and dry weight did not differ between non-irradiated and irradiated twigs, the shoots of non-irradiated nodes were significantly shorter. The non-irradiated plants showed higher amounts of chlorophyll a, b, and lycopene, and a lower concentration of β-carotene. Furthermore, the chlorophyll a/b ratio was lower than that of the control (irradiated) plants. Beyond corroborating our hypothesis, our results suggest that, when light is blocked on the node of C. racemosa, the holoparasite responds in a way similar to that of autotrophic plants under shaded conditions. Our findings suggest a new and previously unacknowledged role for the node of C. racemosa, which would be supporting shoot elongation through photosynthesis.

RESUMO A dermatite atópica canina (DAC) é uma doença inflamatória crônica da pele e tem alta frequência entre as doenças dermatológicas. A interação de fatores genéticos, pele e condições ambientais afetam a expressão da doença, desenvolvendo uma patologia complexa. O tratamento multimodal atual apresenta inúmeros efeitos adversos e variações em sua eficácia e segurança, demonstrando a necessidade de desenvolver recursos terapêuticos seguros e eficazes para pacientes com DAC. As células-tronco mesenquimais (CTM) são células multipotentes, com características especiais, como auto renovação, propriedades imunomoduladoras e desdiferenciação, tornando-se úteis para diversos problemas clínicos. A descoberta do efeito imunossupressor das CTMs sobre as células T abriu o potencial para novas perspectivas com sua utilização como agente terapêutico para doenças imunológicas, como a DAC. O escasso número de pesquisas utilizando o MSC como tratamento para DAC resulta no desconhecimento dos benefícios e dos possíveis protocolos a serem seguidos para a utilização dessa terapia celular. Nesta revisão, destacamos os estudos clínicos e os potenciais mecanismos biológicos dos efeitos da terapia celular baseada em MSC que atenuam a dermatite atópica canina em comparação com o tratamento convencional, podendo levar a uma melhora segura da condição clínica do animal em um curto período, sem causar efeitos adversos.

ABSTRACT Canine atopic dermatitis (CAD) is a chronic inflammatory skin disease and has a high frequency among dermatological diseases. The interaction of genetic factors, skin and environmental conditions affect the expression of the disease, developing a complex pathology. Current multimodal treatment has numerous adverse effects and variations in its efficacy and safety, demonstrating the need to develop safe and effective therapeutic resources for patients with CAD. Mesenchymal stem cells (MSCs) are multipotent cells, with special characteristics, such as self-renewal, immunomodulatory properties, and de-differentiation, making them useful for several clinical problems. The discovery of the immunosuppressive effect of MSCs on T cells has opened the potential for new perspectives with its use as a therapeutic agent for immune diseases, such as CAD. The scarce number of research using the MSC as a treatment for CAD result in the lack of knowledge about the benefits and possible protocols to be followed for the use of this cell therapy. In this review, we highlighted the clinical studies and potential biological mechanisms of MSC-based cell therapy effects attenuating canine atopic dermatitis compared to conventional treatment, which might lead to a safe improvement of the animal’s clinical condition in a short period without causing adverse effects.

This study compared the access to oral health in primary care, before and after the beginning of the COVID-19 pandemic in Brazil. An observational study with a cross-sectional ecological design was carried out, using data from the Health Information System for Primary Care (SISAB). Data regarding the number of Oral Health Teams (OHT), Oral Health Coverage in Primary Care (OHC), number of First Programmatic Dental Consultations (FPDC), and number of visits due to dental abscess and toothache were collected. Data were collected by state as consolidated of the first quarter (January to April) of 2019 and of 2020. The median of the difference (MD) and the percentage of variation (%V) were obtained for each variable. Data were compared by Wilcoxon test (α<0,05). An increase in the number of OHT was observed in 25 states (MD=45, %V=6.13%, p<0.001), whilst the OHC increased in 17 states (MD=1.01, %V=1.62%, p=0.035) between the 2019 and 2020. We also verified a significant reduction in the number of FPDC (MD=- 42,806, %V=-38.70%, p<0.001), as well as in the number of visits due to dental abscess (MD=-1,032, % V=-29.04%, p=0.002) and due to toothache (MD=-14,445, %V=-32.68%, p<0.001). Although an expansion of OHT and OHC between 2019 and 2020 was verified, access to oral health in primary care has decreased due to the COVID-19 pandemic.

Este estudo analisou acesso em saúde bucal na atenção básica, antes e após o início da pandemia do COVID-19 no Brasil. Realizou-se um estudo observacional, com delineamento ecológico transversal, que utilizou dados do Sistema de Informação em Saúde para a Atenção Básica (SISAB). Foram coletados dados referentes ao número de Equipes de Saúde Bucal (ESB), Cobertura de Saúde Bucal na Atenção Básica (CobSB), número de Primeiras Consultas Odontológicas Programadas (PCOP), e número de atendimentos devido abscesso dento-alveolar e dor de dente. Os dados foram coletados por estado como um consolidado do primeiro quadrimestre (Janeiro a Abril) de 2019 e 2020. A mediana da diferença (MD) e o percentual de variação (%V) dos valores foram obtidos para cada variável em estudo. Os dados foram comparados por meio do teste Wilcoxon (α<0,05). Um aumento do número de ESB foi observado em 25 estados (MD=45, %V=6,13%, p<0,001), enquanto a CobSB aumentou em 17 estados (MD=1,01, %V=1,62%, p=0,035), entre 2019 e 2020. Observou-se ainda redução significativa no número de PCOP (MD=-42.806, %V=-38,70%, p<0,001), bem como no número de atendimentos devido abscesso dento-alveolar (MD=-1.032, %V=-29,04%, p=0,002) e a dor de dente (MD=-14.445, %V=-32,68%, p<0,001). Embora tenha sido verificada ampliação de ESB e CobSB entre 2019 e 2020, o acesso à saúde bucal na atenção básica foi reduzido devido a pandemia do COVID-19.

Abstract Objective: To compare the offer and use of oral health services in primary care, before and after the beginning of the COVID-19 pandemic in Brazil. Material and Methods: An observational study with a cross-sectional ecological design, using data from the Health Information System for Primary Care. Data regarding the number of Oral Health Teams (OHT), Oral Health Coverage in Primary Care (OHC), number of First Programmatic Dental Consultations (FPDC), and number of visits due to dental abscess and toothache were collected. Data regarding the 26 Brazilian states and Federal District were collected, as consolidated of the first quarter (January to April) of 2019 and of 2020. The median of the difference (MD) and the percentage of variation (%V) were obtained for each variable and were compared by Wilcoxon test (α<0.05). Results: An increase in the number of OHT was observed in 25 states (MD=45; %V=6.13; p<0.001), whilst the OHC increased in 17 states (MD=1.01; %V=1.62; p=0.035) between the 2019 and 2020. We also verified a significant reduction in the number of FPDC (MD=- 42.806; %V=-38.70; p<0.001), as well as in the number of visits due to dental abscess (MD=-1.032; %V=-29.04; p=0.002) and due to toothache (MD=-14.445; %V=-32.68; p<0.001). Conclusion: Although an expansion of OHT and OHC between 2019 and 2020 was verified, the offer and use of oral health services in primary care has decreased due to the COVID-19 pandemic.

RESUMO A influência da salinidade da água no cultivo da tilápia do Nilo com bioflocos foi avaliada por meio de indicadores de qualidade da água, desempenho zootécnico e bem-estar animal. Foram adotados cinco tratamentos, envolvendo salinidades (0, 4, 8, 12 e 16 g L-1) com três repetições. As tilápias foram cultivadas por 90 dias e estocadas em tanques de fibra de vidro (800 L), numa densidade de 30 peixes m-3 e com peso médio inicial de 93,8 ± 0,89 g. A temperatura da água, clorofila-a, sólidos sedimentáveis e sólidos suspensos totais não diferiram entre as salinidades (p>0,05). Os níveis de nitrogênio da amônia total (3,94 mg L-1) e do nitrito (0,82 mg L-1) foram menores na salinidade 0 g L-1, em comparação à maior salinidade (16 g L-1) (p≤0,05). O peso final variou de 256 a 280 g, com uma sobrevivência de 97 a 100% (p>0,05), que resultou em produtividades entre 7,4 e 8,4 Kg m-3 (p≤0,05). A glicose esteve acima do valor indicado como basal (≤60 mg dL-1) no tratamento 16 g L-1 (76 mg dL-1) e o crescimento dos peixes nas salinidades 8 e 12 g L-1 indicou alometria positiva (3,020), comparando-se aos demais tratamentos. Desta forma, constatou-se a possibilidade de cultivar a tilápia do Nilo em salinidades de até 16 g L-1, sem comprometer o desempenho do cultivo.

ABSTRACT The influence of water salinity on the culture of Nile tilapia with biofloc was evaluated using indicators of water quality, zootechnical performance and animal welfare. Five treatments of different salinities were adopted (0, 4, 8, 12 and 16 g L-1) with three replications. The tilapia were cultured for 90 days and stocked in glass fiber tanks (800 L) at a density of 30 fish m-3 with a mean initial weight of 93.8 ± 0.89 g. Water temperature, chlorophyll-a, sedimentable solids and total suspended solids showed no difference between salinities (p>0.05). The levels of total ammonia nitrogen (3.94 mg L-1) and nitrite nitrogen (0.82 mg L-1) were lower at the salinity of 0 g L-1 compared to the highest salinity (16 g L-1) (p≤0.05). The final weight ranged from 256 to 280 g, with a survival rate of 97 to 100% (p>0.05), which resulted in a productivity of 7.4 to 8.4 kg m-3 (p≤0.05). Glucose was above the baseline value (≤ 60 mg.dL-1) in the 16 gL-1 treatment (76 mg dL-1), and fish growth at the salinities of 8 and 12 g L-1 showed positive allometry (3.020) compared to the other treatments. This demonstrated that it was possible to culture Nile tilapia at salinities of up to 16 g L-1, without compromising performance.

ABSTRACT Objectives: To identify the clinical and epidemiological features of interviews with families of potential donors of organs and tissues. Methods: Quantitative research, descriptive, exploratory and documental. The sample was composed of medical records of 93 patients, whose families have been addressed in the years 2012/2013. It conducted data analysis using the statical package “R”. Results: It was observed that 62.4% of approaches have ben performed with families of patients who have become potential cardiorespiratory arrest donor. Parents were the most interviewed family members coming to meet 40% of approaches. The family consent to donation percentage was 51.6%. Eye/corneas globe was the most spolied, coming to represent 87.5%. Knowing tha the potential donor was contrary (in life) donation (46.7%) and ignored his desire in life (33.4%) werw the main family groups for refusal. Conclusion: Identify the clinical and epidemiological elements of family interviews for donation is very important to evaluate the effectiveness of the activities performed in the donation process of the institutions

RESUMO Objetivos Identificar os elementos clínico-epidemiológicos das entrevistas realizadas com familiares de potenciais doadores de órgãos e tecidos. Médotos Estudo quantitativo, descritivo, exploratório e documental. A amostragem foi composta por prontuários de 93 pacientes, cujas famílias foram abordadas nos anos de 2012/2013. Realizou-se análise dos dados por meio do pacote estatístico “R”. Resultados Observou-se que 62,4% das abordagens foram realizadas com familiares de pacientes que se tornaram potenciais doadores pós-parada cardiorrespiratória. Os pais foram os familiares mais entrevistados, chegando a corresponder 40% das abordagens. O percentual de consentimento familiar para doação foi de 51,6%. Globo ocular/córneas foi o mais consentido, chegando a representar 87,5%. Saber que o potencial doador era contrário (em vida) a doação (46,7%) e desconhecer seu desejo em vida (33,4%) foram os principais motivos de recusa familiar. Conclusão Identificar os elementos clínico-epidemiológicos das entrevistas familiares para doação é de suma importância para avaliar a eficácia das atividades desempenhadas no processo de doação das instituições.

RESUMEN Objetivos Identificar los elementos clínicos y epidemiológicos de las entrevistass con las familias de potenciales donantes de órganos y tejidos. Métodos Estudio cuantitativo, descriptivo, exploratorio y documental. La muestra consta de 93 registros de pacientes, cuyas familias fueron abordadas en los años 2012/2013. Se realizó un análisis de los datos mediante el paquete estadístico “R”. Resultados Se observó que el 62,4% de los enfoques se han realizado con familiares de los pacientes que se han convertido en potenciales donantes post-parada cardiorrespiratoria. Los padres fueron los miembros de la familia más entrevistados (40%). El porcentaje de donación de la familia fue del 51,6%. Globo del ojo/córneas fue el más consentido, llegando a representar 87,5%. Saber que el donante potencial era contrario (en vida) a la donación (46,7%) y desconocer su deseo en vida (33,4%) fueron las principales razones de la negativa de la familia. Conclusión Identificar los elementos clínicos y epidemiológicos de entrevistas familiares para la donación es muy importante para evaluar la eficacia de las actividades realizadas en el proceso de donación de las instituiciones.

RESUMO: O azevém anual (Lolium multuflorum Lam) é uma das plantas forrageiras de clima temperado mais cultivadas no mundo. Apesar disso, há poucas informações sobre o processo de alongamento de colmos para essa espécie durante seu crescimento vegetativo. Tal informação seria útil para o manejo mais adequado desta espécie. Assim, o objetivo do presente trabalho foi avaliar o acúmulo de forragem e as taxas de alongamento de colmos em pastos de azevém anual submetidos à lotação intermitente. O delineamento experimental foi completamente casualizado em arranjo factorial 2x2, com três repetições. Os tratamentos consistiram na combinação de duas alturas em pré-pastejo (15 e 25cm), associadas com duas alturas de resíduo (4 e 8cm). A maior taxa de acúmulo de forragem foi observada nos pastos manejadas com 25cm, associadas com alturas de resíduo de 8cm. Entretanto, a taxa de acúmulo de folhas foi similar para pastos manejados com 8cm de resíduo, independente da altura em pré-pastejo (15 ou 25cm). A taxa de alongamento de colmos em perfilhos de azevém anual aumentaram rapidamente quando os pastos atingiram uma altura de 18cm, condição onde havia uma relativa baixa competição por luz, sugerindo que: 1) alturas em pré-pastejo entre 18 e 20cm em pastos de azevém anual poderia ser usada como um limite máximo de altura quando o objetivo for minimizar a contribuição de colmos na forragem acumulada; e 2) parece que o alongamento de colmos é um processo inevitável (independente de competição por luz) e isso poderia estar relacionado com a força necessária de colmos, pseudocolmos e bainhas para suportar o peso de folhas cada vez maiores e mais pesadas.

ABSTRACT: Italian ryegrass (Lolium multiflorum Lam) is one of the most cultivated temperate annual forage crop around the world. Despite that, there is little information about the process of stem elongation during its vegetative growth. This information would be useful for grazing management purposes. Thus, the aim of this study was to assess the herbage accumulation and stem elongation rates in annual ryegrass pastures submitted to intermittent stocking. The experimental design used was a completely randomized 2x2 factorial with three replications. The treatments were defined by the combination of two pre-grazing heights (15 and 25cm) associated with two post-grazing heights (4 and 8cm). The herbage accumulation rates were higher in pastures with 25cm pre-grazing associated with post-grazing heights of 8cm. However, leaf production rate was only affected by post-grazing height, with higher values being recorded for stubbles heights of 8cm, regardless of pre-grazing height. Stem elongation rates in annual ryegrass tillers increased rapidly when pastures reached around 18cm, a condition where there was a relatively low competition for light, suggesting that; 1) pre-grazing heights of 18 to 20cm in annual ryegrass could be used as a upper limit for grazing management purposes whether the objective is to minimize stem contribution in accumulated herbage and 2) It seems that stem elongation is as inevitable process (independently of competition for light) and that this could be related to the necessary strength of stems, pseudostems and sheaths to support larger and heavier leaves.

Introduction: The essential oil Mentha x villosa (MVEO) has a wide range of actions, including antibacterial, antifungal, antiprotozoal and schistosomicidal actions. The present study aimed to investigate the ultrastructural changes of MVEO on the tegument of adult Schistosoma mansoni. Materials and Methods: Different concentrations of MVEO were tested on S. mansoni adult worms in vitro. Ultrastructural changes on the tegument of these adult worms were evaluated using scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Results: The MVEO caused the death of all worms at 500 μg mL-1 after 24 h. After 24h of 500 μg mL-1 MVEO treatment, bubble lesions were observed over the entire body of worms and they presented loss of tubercles in some regions of the ventral portion. In the evaluation by TEM, S. mansoni adult worms treated with MVEO, 500 μg mL-1, presented changes in the tegument and vacuoles in the syncytial matrix region. Glycogen granules close to the muscle fibers were visible. Conclusion: The ability of MVEO to cause extensive ultrastructural damage to S. mansoni adult worms correlates with its schistosomicidal effects and confirms earlier findings with S. mansoni.

RESUMO: O presente trabalho avaliou o desempenho do camarão marinho Litopenaeus vannamei alimentado com dietas de diferentes níveis protéicos em sistema de bioflocos com e sem a adição de probiótico. Foi adotado um delineamento experimental inteiramente casualizado com arranjo fatorial 4×2, com quatro níveis de proteína na dieta (20, 25, 30 e 35% PB), como primeiro fator (P20, P25, P30 e P35), e a adição de probiótico na água de cultivo, como segundo fator (P20Pro, P25Pro, P30Pro e P35Pro). Foram utilizados 24 tanques em fibra de vidro (800 L volume útil) estocados com 300 camarões m-3 (peso inicial 1,55±0,01 g). As variáveis de qualidade da água foram mensuradas periodicamente e não apresentaram diferença estatística, exceto o teor de nitrito, influenciado significativamente (P<0,05) pelos níveis de proteína. Após 50 dias de cultivo o peso médio final dos camarões foi de 7,2±0,4 g (P≥0,05) entre os tratamentos. A interação entre os níveis protéicos e a adição de probiótico influenciaram significativamente (P<0,05) na sobrevivência (70,5-90,0%) e na biomassa final (1,3-2,0 Kg m-3). Dessa forma, em cultivo intensivo de L. vannamei, com utilização de bioflocos como fonte de alimento suplementar, é possível reduzir os níveis de proteína da ração de 35 para 25% sem comprometer o desempenho zootécnico dos camarões e a qualidade da água.

ABSTRACT: This study aimed to evaluate the culture of Litopenaeus vannamei marine shrimp fed with different protein levels diets in heterotrophic systems with and without probiotic addition. It was adopted a completely randomized design with 4x2 factorial arrangement, using four dietary protein levels (20, 25, 30 e 35% CP), as the first factor (P20, P25, P30, and P35), and probiotic addition in the water, as the second factor (P20Pro, P25Pro P30Pro e P35Pro). For this were used 24 fiberglass tanks (800 L working volume) stocked with 300 shrimp m-3 (initial weight 1.55±0.01 g). Water quality parameters were analyzed periodically and showed no significant differences, except nitrite that was influenced by the protein levels (P<0.05). After 50 culture days, shrimp final weight averaged 7.2±0.4 g (P≥0.05). The interaction of protein levels vs. probiotic addition influenced significantly (P<0.05) the survival (70.6 to 90.0%) and final biomass (1.3-2.0 Kg m-3). In Litopenaeus vannamei intensive culture with the utilization of biofloc as the supplemental food, it is possible to reduce the protein levels of feed from 35 to 25%, without compromising the shrimp growth performance and water quality of the culture.